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Peptide

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Educational peptide reference — research use only.

Research & educational use only

For laboratory and educational research only. Not for human or veterinary consumption. This is not medical advice. Always follow applicable laws and consult qualified professionals.

The calculator performs unit math for research reference. It must not be used to plan or guide dosing in humans or animals. Verify all figures independently in your lab protocol.

FOXO4-DRI

A D-retro-inverso peptide studied in senescent-cell clearance research models.

Half-life (approx.)
~hours (approx., senolytic research)
Diluent
Bacteriostatic water (0.9% benzyl alcohol)
Common vials
10 mg

Half-life figures are literature approximations for educational reference — not pharmacokinetic advice.

Overview

FOXO4-DRI is a D-retro-inverso peptide that disrupts FOXO4-p53 interaction to selectively induce apoptosis in senescent cells (senolytic research). Preclinical work in mice examines tissue rejuvenation markers after senescent-cell clearance. Senolytic D-retro-inverso peptide targeting FOXO4-p53 interaction in senescent cells.

Structure & identity

D-retro-inverso peptide FOXO4-DRI (senolytic)

Sequence / structure
D-retro-inverso peptide FOXO4-DRI (senolytic)

Status: Preclinical senolytic research stage.

Mechanism

Preclinical senolytic research stage.

D-retro-inverso peptide disrupts FOXO4-p53 interaction in senescent cells, triggering apoptosis. Selective apoptosis of senescent cells reduces SASP burden in progeroid mouse models.

Studies & clinical programs

  • Progeroid mice

    Published research models

    • Peer-reviewed literature documents endpoints under Progeroid mice experimental designs.
  • Senescent cell p16 assays

    Published research models

    • Peer-reviewed literature documents endpoints under Senescent cell p16 assays experimental designs.
  • Hair regrowth models

    Published research models

    • Peer-reviewed literature documents endpoints under Hair regrowth models experimental designs.

Research models in literature

  • Progeroid mice
  • Senescent cell p16 assays
  • Hair regrowth models

Literature highlights

  • D-retro-inverso peptide disrupts FOXO4-p53 in senescent-cell clearance models.
  • Senolytic research examines p16+ cell burden and tissue rejuvenation markers.
  • Progeroid and chemotherapy-induced senescence models used as preclinical contexts.

Key targets & pathways

FOXO4Senescent cellsp16INK4a+ cellsSASP cytokines

Research areas

SenolyticsCellular senescenceAging intervention

Routes in research literature

Subcutaneous

Also known as

FOX04ProxofimFOX04-DRIFOXO4 DRI

Stability & storage phases

PhaseConditionGuidance
LyophilizedSealed vial, refrigerated (2–8 °C)Intact lyophilized cake or powder is typically stable for months to years per published stability data; protect from moisture, light, and repeated freeze-thaw of the dry vial.
ReconstitutedBacteriostatic water (0.9% benzyl alcohol), refrigeratedMost aqueous peptide solutions remain usable for approximately 2–4 weeks refrigerated; verify published stability data and label with reconstitution date.
Working aliquotsPre-drawn syringes or microtubes, frozen (−20 °C)Aliquot promptly after mixing to limit freeze-thaw cycles on the main vial; thaw once and use to reduce protease-mediated degradation.

Stability windows are formulation-dependent — verify published data and your lab SOP.

Reconstitution reference table

Vial (mg)Diluent (mL)mcg/mLUnits @ 100 mcgUnits @ 250 mcgUnits @ 500 mcg
1025000.02510

U-100 insulin syringe scale (100 units = 1 mL). Illustrative only — not dosing guidance.

Reconstitution steps

  1. Allow vial to reach room temperature (15–30 min)
  2. Swab rubber stopper with alcohol prep pad
  3. Draw calculated bacteriostatic water into syringe
  4. Inject diluent slowly down vial wall — do not spray directly onto cake
  5. Gently swirl until fully dissolved — do not shake vigorously
  6. Label with date, concentration, and diluent volume
  7. Refrigerate and use within your lab stability window

Typically reconstituted with bacteriostatic water per protocol.

Laboratory record checklist

  • Compound identity recorded in lab notebook (name, lot, preparation date)
  • Analytical identity cross-checked against published sequence or structure
  • Potency or concentration documented from analytical certificate when available
  • Purity or HPLC data filed when provided with research material
  • Appearance noted: intact lyophilized cake or uniform powder
  • Sterility / endotoxin report archived when available
  • Storage temperature applied immediately per published stability guidance