Peptide
Reference library
Educational peptide reference — research use only.
Research & educational use only
For laboratory and educational research only. Not for human or veterinary consumption. This is not medical advice. Always follow applicable laws and consult qualified professionals.
The calculator performs unit math for research reference. It must not be used to plan or guide dosing in humans or animals. Verify all figures independently in your lab protocol.
TB-500 (TB4)
Studied in preclinical models related to cell migration and tissue repair.
- Half-life (approx.)
- ~hours (approx.)
- Diluent
- Bacteriostatic water (0.9% benzyl alcohol)
- Common vials
- 2, 5, 10 mg
Half-life figures are literature approximations for educational reference — not pharmacokinetic advice.
Overview
TB-500 is a synthetic fragment of thymosin beta-4 (LKKTETQ) studied for actin sequestration, cell migration, and angiogenesis in wound-healing models. Often researched alongside BPC-157 in combination protocols for tissue-repair endpoints. Synthetic thymosin β4 fragment promoting actin dynamics and cell migration at injury sites.
Structure & identity
Thymosin β4 fragment — 7 aa LKKTETQ (research synthetic)
- Sequence / structure
- Ac-Nle-Asp-His-Lys-Ser (thymosin β4 active region fragment)
Mechanism
TB-500 (thymosin β4 fragment) regulates G-actin pools to facilitate cytoskeletal remodeling and cell migration in repair research.
Thymosin β4 fragment sequesters G-actin, promoting cell migration and angiogenesis at injury sites. G-actin sequestration enables cytoskeletal remodeling in corneal, cardiac, and tendon repair models.
Studies & clinical programs
Thymosin β4 fragment research
Corneal and cardiac injury rodent models
- Actin sequestration promotes cell migration and angiogenesis at injury sites.
- Wound closure and repair endpoints in published preclinical protocols.
Research models in literature
- Corneal wound healing
- Cardiac repair rodent models
- Tendon injury
Literature highlights
- Thymosin β4 fragment promotes actin sequestration, cell migration, and angiogenesis at injury sites.
- Corneal wound healing and cardiac repair rodent models feature prominently in literature.
- Frequent research partner for BPC-157 in recovery combination studies.
Combination research notes
Paired with BPC-157 in mixes and GLOW/KLOW blends.
Key targets & pathways
Research areas
Routes in research literature
Also known as
Stability & storage phases
| Phase | Condition | Guidance |
|---|---|---|
| Lyophilized | Sealed vial, refrigerated (2–8 °C) | Intact lyophilized cake or powder is typically stable for months to years per published stability data; protect from moisture, light, and repeated freeze-thaw of the dry vial. |
| Reconstituted | Bacteriostatic water (0.9% benzyl alcohol), refrigerated | Most aqueous peptide solutions remain usable for approximately 2–4 weeks refrigerated; verify published stability data and label with reconstitution date. |
| Working aliquots | Pre-drawn syringes or microtubes, frozen (−20 °C) | Aliquot promptly after mixing to limit freeze-thaw cycles on the main vial; thaw once and use to reduce protease-mediated degradation. |
Stability windows are formulation-dependent — verify published data and your lab SOP.
Reconstitution reference table
| Vial (mg) | Diluent (mL) | mcg/mL | Units @ 100 mcg | Units @ 250 mcg | Units @ 500 mcg |
|---|---|---|---|---|---|
| 2 | 2 | 1000.0 | 10 | 25 | 50 |
| 5 | 2 | 2500.0 | 4 | 10 | 20 |
| 10 | 2 | 5000.0 | 2 | 5 | 10 |
U-100 insulin syringe scale (100 units = 1 mL). Illustrative only — not dosing guidance.
Reconstitution steps
- Allow vial to reach room temperature (15–30 min)
- Swab rubber stopper with alcohol prep pad
- Draw calculated bacteriostatic water into syringe
- Inject diluent slowly down vial wall — do not spray directly onto cake
- Gently swirl until fully dissolved — do not shake vigorously
- Label with date, concentration, and diluent volume
- Refrigerate and use within your lab stability window
Commonly reconstituted with 1–3 mL bacteriostatic water.
Laboratory record checklist
- Compound identity recorded in lab notebook (name, lot, preparation date)
- Analytical identity cross-checked against published sequence or structure
- Potency or concentration documented from analytical certificate when available
- Purity or HPLC data filed when provided with research material
- Appearance noted: intact lyophilized cake or uniform powder
- Sterility / endotoxin report archived when available
- Storage temperature applied immediately per published stability guidance